Sentence examples for bead solution was then from inspiring English sources

The bead solution was then centrifuged at 3600 rpm for 15 minutes and re-suspended in water.

The protein bead solution was then incubated at room temperature in a tube rotator (Miltenyi Biotec) for 2 hours followed by three subsequent centrifugation (3600 rpm for 15 minutes) and re-suspension steps (PBS, pH 7.4) to remove unbound protein.

The bead solution was then placed as droplet in a circular shallow chamber created by varnish or silicone tape [ 19].

The Alexa488/InlA-bead solution was then subjected to three centrifugation (3600 rpm for 15 minutes) and re-suspension steps (PBS, pH 7.4) to remove any free Alexa488.

FITC/InlA-bead solution was then subjected to three centrifugation (3600 rpm for 15 minutes) and re-suspension steps (PBS, pH 7.4) to remove any free FITC.

The CaCl2 solution was then removed and the beads were rinsed twice with sterile distilled water.

The captured target solution was then added to the beads and rotated for 30 minutes at room temperature.

The supernatant solution was then added back to the beads which were then re-incubated as above prior to the next sampling.

After the final wash (Step 8 of Fig. 3), a trypsin solution was added directly to the Co-Talon magnetic beads, and the slurry was gently mixed and incubated at 37 C for 5 min. After 5 minutes, the beads were separated from the supernatant with a magnet; the solution was then collected and incubated at 37 C for 5 hours to digest all proteins that came off the beads.

The protein solution was then applied to neutravidin-coated agarose beads to facilitate protein pull-down.

The mixture containing FITC-fibronectin and unreacted FITC (i.e. still free in solution) was then used for conjugation to 2 µm carboxyl terminated polystyrene beads.