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Results for the sheet metal materials considered confirm quantitatively that the binder angle significantly influences the draw bead pulling force.
Taken together, results reported here indicate that in response to repetitive load transients the reinforcement response in the HASM cell is peculiar to non-homogeneous cell deformations, as would occur during repetitive bead pulling or needle poking, but fails to scale up to the case of repetitive homogeneous cell stretch, whether isotropic or anisotropic.
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In the absence of plasminogen, the bead pull down of cell wall protein extracts failed to yield detectable protein.
After induction, cells were lysed and proteins were purified by IgG sepharose bead pull down, followed by cleavage with protease 3C.
Take the last bead, pull it higher on the wire than the rest, and start to tie it as in step 1, but instead of pulling it all the way, place a knitting needle into the loop, and pull towards the other beads.
Designate one of your bead colors as color A and one as color B. Thread three (3) color A beads onto your thread followed by three (3) of color B. Take your needle and, starting at your first color A bead, pull your thread through all three (3) color A beads.
Prep1-GST and 4EHP-GST beads pulled down 35S-Met-4EHP and, respectively, 35S-Met-Prep1 (Fig. 3B).
m7GTP beads pull down more 4EBP1 protein in RBS cells compared to corrected cells (Fig. 1c).
Control beads pulled down no detectable Met protein from either PC3 or C4-2 lysases, assayedyed by both silver staining and immunoblotting.
It would also be interesting to know whether neuronal cell bodies, and not just their axons, are susceptible to H1 toxicity in the collagen gel co-culture; and to confirm that H1-antibody-coated beads pull off the neurotoxic activity.
Thread the wire through a few of the ending beads, pull tight, close the crimper by squeezing it with the cutters, and then trim the excess wire.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com