Bead probing with optical tweezers provides a new, nondestructive method that may have wide applicability for studying the effects of growth conditions, immune components, and drugs on capsular properties.
Such a deviation from equilibrium has previously been observed in the cytoplasm of highly motile amoebae in experiments with phagosomal probes [25], and in a system using an active actin gel recomposed outside the cell and micrometric bead probes [20].
Species-specific and pestivirus-common oligonucleotide probes were designed to the 5′ UTR region and conjugated to individual color-coded Luminex carboxy beads (probe beads).
Samples were added to each dsDNA-bead probe and incubated at 4 °C for 1 h and mixed end-over-end.
For optimal assay performance it is crucial to apply an easy, efficient and robust bead-probe conjugation protocol.
The bead-probe-fragment complex was washed three times each for 5 minutes with 400 μl non-stringency washing buffer (1 × TE + 1 M NaCl) at room temperature.
We recently characterized HAmo SINE and its partner LINE in silver carp and bighead carp based on hybridization capture of repetitive elements from digested genomic DNA in solution using a bead-probe [ 1].
Recently, we characterized a couple of SINE and LINE, designated HAmo SINE and its partner LINE, in silver carp and bighead carp based on hybridization capture of repetitive elements from digested genomic DNA in solution using a bead-probe [ 1].
In the middle, F or I was used to represent the internal SINE sequence or full-size sequence obtained from the bead-probe capture (F) or internal-primer-SINE PCR (I), respectively.
Two bead sizes, 100 and 250 nm, are investigated along with a highly efficient, rapid, robust, and stable conjugation chemistry relying on the avidin biotin interaction for bead-probe attachment.
A: Distoechodon tumirostris B: Schizothorax meridionalis C: Barbodes huangchuchieni A rapid bead-probe capture technique based on magnetic separation was used to isolate full-size of HAmo SINE copies from unknown genomes as described previously [ 1].
