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The designed aptamer beacons were further successfully applied for RT imaging in HIV-1 integrated U1 cells.
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As proof-of-principle, this novel DNA-protein hybrid molecular beacon was further applied for FA bioassay using DNAzyme-Pb2+ as a model sensing system.
A catalytic and molecular beacons strategy is further adopted to amplify the electrochemical signal detection achieved by cycling and regenerating the 8-17 DNAzyme to realize enzymatic multiple turnover, thus one DNAzyme can catalyze the cleavage of several hairpin-structured substrates, which improves the sensitivity of the newly designed electrochemical sensing system.
In HC-MAC (Jia and Zhang 2007) time is divided into beacon intervals, and each beacon interval is further divided into three phases: channel selection, sensing, and data transmission.
Isolates with mutations in katG detected by the wild-type probe were further tested with another molecular beacon that specifically targeted katG S315T (AGC ACC).
Based on the positive results indicated in [11, 57], it was concluded that adaptive beaconing should definitely be further explored.
The beacon frame overhead can be further reduced by dropping IP and UDP protocols and using MAC frames only, but this would complicate the clustering algorithm as some features of the dropped protocols would have to be implemented separately.
Both beacons were manually activated.
Two beacons were triggered in March 2013.
Molecular beacons were modified and designed.
Molecular beacons were obtained from Integrated DNA Technologies Coralvillee, IA).
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