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Molecular beacons were designed to hybridize with either type of mtDNA molecule, allowing real-time detection during PCR amplification.
The molecular beacons were designed to complement the wild-type codon 460 or the mutant sequence arising from a single base-pair difference (point mutation).
FRET-based molecular beacons were designed as poly(A -targeting probes to be oligonucleotides thA -targeting Cy5 and Cy3 fluorescent dyes at the strand ends and a probes)-torgeting sequence inside the strand.
Shared-stem molecular beacons were designed by utilizing one or both of the stem sequences as complementary portion to the targets [19].
Molecular beacons were designed by D. Bratu and synthesized by M. Mhlanga & C. Gouyette at the Plateforme de Synthèse d'Oligonucléotides Longs à Haut Debit (Institut Pasteur) as described in [27], [74].
To visualize Oct-4 mRNA in live stem cells, molecular beacons were designed, synthesized and validated, and the detection specificity was confirmed using control studies.
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A dual-signal amplification method based on two molecular beacons was designed for human hemochromatosis (HFE) gene detection.
Two aptasensors based on graphene oxide (GO) and molecular beacon were designed for the detection of L-tryptophan (L-Trp) using L-Trp aptamer (Trp3a-1).
A pair of primers and a probe (molecular beacon) were designed that are specific for the recognition of a highly conservative 5′-non-coding region (5′-NCR) in HCV genome.
In FMCA, both the TaqMan and shared-stem molecular beacon were designed in the same way as in real-time PCR.
The PCR primers and the target recognition sequence of the molecular beacon were designed to hybridize on conserved regions from all the genetic subtypes within the M group based on a comprehensive DNA sequence alignment from published HIV-1 sequences (Table 1 and Figure 1).
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