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Cellular uptake and cytoplasmic release of siRNA over time were quantified by measuring the fluorescence intensities of fluorescently-labeled siRNAs and molecular beacons using flow cytometry.
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Specifically, we designed molecular beacons to target Oct-4 mRNA in live EC cells in addition to targeting SSEA-1 protein using dye-labeled antibodies, and used flow cytometry to demonstrate the effectiveness in processing a large number of cells.
Use flow tests.
Beacons use a combination of both software and hardware.
Primers and molecular beacons used in the IS481 and IS1001 molecular beacon (MB) assays are previously described [ 8].
Each ship's homing beacon used a distinctly different frequency.
Beacons are used to discover and maintain neighbour relationships [3 5].
A beacon antenna is proposed using in beacon device of digital flight data recorder for harsh environment.
The genotyping experiments were performed in the ABI 7700 using beacons labeled with either fluorescein or JOE, and in the Lightcycler using a fluorescein labeled beacon.
The beacon is used to synchronize BMSs with a BANC.
The second type of probe, shared-stem molecular beacon, was used when high sensitivity was required.
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