Beacon's version, which is directed and choreographed by Andy Blankenbuehler ("In the Heights"), with a book by Jeff Whitty ("Avenue Q"), and music and lyrics by Lin-Manuel Miranda ("In the Heights"), Tom Kitt ("Next To Normal"), and Amanda Green ("High Fidelity"), had a run earlier this year in Atlanta and is set to begin a 10-city national tour in Los Angeles on Oct. 30.
The primers were designed with Beacon Designer, version 7.7 (Premier Biosoft International, Ltd., Palo Alto, CA, USA).
Specific primers for each gene were designed using Beacon Designer version 7.0 (Premier Biosoft, Palo Alto, CA) (Additional file 12).
The primers for PCR were designed using software Beacon Designer Version 7.9 (Premier Biosoft International, Palo Alto, CA, USA).
Primers were designed using Beacon Designer, version 7.2, selecting for a primer annealing temperature of 55 °C and amplicon length of <100 bp.
It is a signature symbol and a beacon, golf's version of a lighthouse — sometimes barely visible from the tee box but ever more noticeable as the golfer draws closer.
All primers and probes [44] were designed with Beacon Designer 2 (version 2.06) software (Premier Biosoft International) and synthesized by MWG Biotech (Ebersberg, Germany).
Briefly, pairs of PCR primers were designed using the Beacon Designer software (version 2, California, USA).
PCR primers (Additional file 3) were designed using the Beacon Designer software (version 7.0) (Premier Biosoft International, Palo Alto, California, USA).
PCR primers were designed using Beacon Designer software (version 7) and listed in Additional file 10: Table S7.
