In a nutshell, the molecular beacon based PCR assay developed in the present study addresses to a large extent the need of a simple, cost effective, easy to use, highly sensitive, and specific nucleic acid amplification assay, a step forward towards developing point-of-care diagnostics for low resource settings [ 21, 22].
In this work, we develop a fluorescent molecular beacon based on the DNA-templated silver nanoclusters (DNA-Ag NCs).
For the localisation aspects a cost effective infrared beacon based system is presented for 2D applications.
In this research, a "token" is HMACSHA1 generated by a Beacon based on UUID and Time.
A double-molecular beacon based assay (DMB) was developed for the determination of porcine circovirus 2 (PCV2).
Location verification schemes for detecting false position reports may be beacon based or sensor based.
Linear velocity of sweeping beam on the sensor array can be interpreted as the distance from a beacon based on an equation.
In this study, specific primers and modified molecular beacon probes of nine specific virulence genes, whose 5′end were added with homo tail sequence, were designed; and a two-tube modified molecular beacon based multiplex real time PCR (rtPCR) assay for the identification of five Escherichia coli pathotypes, including ETEC, EAEC, EPEC, EHEC and EIEC was developed and optimized.
MRE beacon based on fluorescent signals was developed and generated strong signal at spore concentrations greater than 30,000 spores/mL.
In brief, the molecular beacon based PCR assay described here meets the essential requirements for use in resource limited settings.
