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Although consistency of protein content was confirmed with Coomassie blue-stained actin, all Western blot signals were evaluated and found to be within the linear range of detection.
Cycle number was empirically determined to be within the linear range of the assay for each primer pair used.
First and foremost, identification of outlier exons requires their transcript expression level to be within the linear detection range of the exon array, which is determined by their transcript expression level as well as the hybridization efficiency and specificity of the probe sets involved.
The exposure times were kept equal for all the images and selected for their intensities to be within the linear range of the detectors.
Albitiazolium entry into isolated P. falciparum parasites was measured after 10 min of incubation in order to be within the linear phase of transport.
The 5 wt % gels were tested at 1 rad/s and 1% strain in order to be within the linear viscoelastic regime for this formulation.
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The thereby acquired HRP signals are within the linear range of the western blot as demonstrated by plotting the obtained quantified signals onto the graph of the standard curve.
To additionally ensure that the quantified western blot signal is within the linear range of detection, we generated standard curves using a dilution series of the respective ubiquitination reaction, which was incubated for 180 min at 30 °C.
Densitometric readings made with a computer program (NIH Image version 1.59) were confirmed as being within the linear range by processing blots with various concentrations (1 10��μg protein) of the same control sample at the time of the assay.
All the values found were within the linear range of the methods, recovery 102%.
All samples were within the linear interval of the standard curve.
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