For the star camera and accelerometer data, the calibration parameters have been well determined and used in data generating and processing.
Considering the predominant affinity of these somatostatin analogues for the same receptor subtype, the sst2, the receptor 'saturation' after treatment with 177Lu-octreotate may very well be determined using 68Ga-DOTATOC PET.
Then, 100 μL of 2-propanol with 0.04 N HCl was added to each well, and UV absorption for each well was determined using a Synergy II plate reader at 570 and 630 nm.
The level of fluorescence in each well was determined using a fluorometer (Spectra Max GeminiXS), and the data were analysed using SOFTmax PRO (Molecular Devices).
TMB Stop Solution was added (0.1 mL/well; KPL, cat# 50-85-06) and the OD450 for each well was determined using a Victor3 plate reader (Perkin Elmer).
Reactions were stopped using 100 μl of Stop Solution and the absorbance of each well was determined using a microplate reader set to 450 nm with reference wavelength of 630 nm.
After washing, plates were incubated with color reagent (hydrogen peroxide chromogen mix) for 30 min. Optical density of each well was determined using a microplate reader set to 450 nm.
The absorbance of each microplate well was determined using a microplate reader (Versa Max, Molecular Devices) (λ 490 nm), and p24 amount was calculated using an HIV-1 p24 antigen standard curve.
The cells in culture were then co-incubated with various doses of rTRAIL for 48 h and the cell viability of each well was determined using a 3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide (MTT) assay [ 14].
The fluorescent signal of the mCherry_PGRP-SA was detected using a 532 nm laser in a Fuji FLA-5100 reader (FUJIFILM Life Science) and the fluorescence intensity of each well was determined using ImageJ software (Abràmoff et al., 2004).
