Definitely, it is well analyzed.
The cell number in each well was analyzed using Celigo™ adherent cell cytometer (Cyntellect Inc .. Every well containing single cells was photographed digitally, and the cells were counted using the Celigo software (1.3).
Approximately 1 × 10 live cells from each transfected well were analyzed using an LSRII flow cytometer (BD Bioscience) running FACSDiva software.
After washing each well three times with water, the fluorescence of each well was analyzed using a Spectramax M5 plate reader (Molecular Devices, Sunnyvale, CA) with excitation 630 nm, emission 697 nm, and cutoff 695 nm, background corrected by subtracting the mean signal from empty wells and normalized to the mean value of untreated wells.
To test the construct validity of the Brazilian CPQ11 14, associations between the scores of each domain, global oral indicators and overall well-being were analyzed using Spearman's correlation coefficient.
Associations between the scores on each domain and the respondents' global rating of oral health and overall well-being were analyzed using Spearman's correlation coefficient to test the questionnaire's construct validity.
Four images per well were analyzed using a neurite outgrowth module (Molecular Devices, Sunnyvale, CA).
Stained trans-wells were analyzed using LEICA, TCS SP2 confocal microscope (Leica, Heerbrugg, Switzerland).
The optical density of the wells was analyzed using a plate reader at wavelength of 405 nm.
After the denaturation at 94°C for 5 min followed by incubation on ice for 5 min, the 96-well plate was analyzed using 3700 DNA Analyzer (Applied Biosystems, Foster, CA).
