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These bioinformatic analyses suggest that the RBD forms a stable obligate homodimer, while the ED helix-helix dimer (although predominant over other interfaces) may only be weak and transient.
However, we currently lack reliable methods to identify, without bias, enzymes that regulate the addition and removal of PTMs, as interactions between PTMs and their regulating enzymes can be weak and transient, thereby limiting the applicability of conventional biochemical 'pull-down' methods.
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Often, such interactions are weak and transient, and the complexes do not easily crystallize.
The activation of RhoA may help in maintaining normal stress fibers in podocytes, but this phenomenon was weak and transient.
The interaction of the Arf GAP and of the transport protein sec-23-like with LmjMCA was weak and transient, not preventing LmjMCA self-interaction.
Having found that IFT inhibition by three independent methods failed to affect SSTR3 movements, we hypothesized that the coupling between single SSTR3 molecules and IFT trains is weak and transient.
While anisomycin-exposure leads to a strong and sustained activation of the p38 and MK2 kinases, thereby increasing the phosphorylation of CREB [ 27, 28], mitogen-induced activation of p38 is weaker and transient, but is required for cyclin D1 expression.
Abbas is weak and acts weaker.
Nevertheless, binding of CAR to α-catenin may not be excluded entirely, as it might be weak and/or transient and not be detectable biochemically.
While the binding of the IFNAR2 receptor to interferon is of high affinity and well characterized, the binding to IFNAR1 is weak, transient, and poorly understood.
Prior to LFA-1 activation, the interaction of 2C TCRs with the cognate pMHCs may be too weak and transient to establish stable pMV absorption.
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