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Sequences within the amplified, concatemeric DNA product can then be visualized with fluorescently-labeled, complementary oligonucleotide probes.

F-actin was visualized with fluorescently-labeled Phalloidin (Invitrogen).

PSD95 and its GAPDH loading control (Abcam) were visualized with fluorescently-labeled secondary antibodies (LI-COR; Lincoln, NE) using an Odyssey Infrared Imager (LI-COR) for quantitation and analysis.

Primary antibody staining was visualized with fluorescently labeled secondary antibodies and confocal laser-scanning microscopy with standard green or red filters.

Primary antibodies were visualized with peroxidase-labeled anti-mouse or anti-rabbit IgG and chemiluminescence detection.

Bound anti-NeuN or anti-nestin antibodies were visualized with Cy3-labeled anti-mouse IgG antibodies (1∶1000).

The bands were visualized with HRP-labeled goat anti-rabbit antibody and enhanced chemiluminescence (Pierce, Rockland, IL, USA).

Proteins were visualized with horseradish peroxidase-labeled secondary antibody (Amersham Biosciences) using the SuperSignal system (Pierce) as substrate.

The proteins were then subjected to SDS-PAGE and fluorescently tagged proteins were visualized with a fluorescence imager.

Primary antibodies were visualized with the appropriate Alexa-labeled secondary antibody from Invitrogen.

Primary antibodies were visualized with the appropriate covalently Alexa-labeled secondary antibody from Invitrogen.