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Marco Gallio, who led the study, explained: "Our results show we can detect a specific pattern of activity between neurons in the brain, recording instantaneous exchanges between them as persistent signals that can later be visualized under a microscope".
As shown in Figure S1, EV71-GFP-infected 293-hSCARB2 cexpresspress GFP and can be visualized under a fluorescence microscope.
The cells infected by EV71-GFP virus express GFP and can be visualized under a fluorescence microscope or can be checked by flow cytometry because the GFP will be translated during virus replication.
The culture was propagated at 60°C until the spheroplast morphology could be visualized under a light microscopy (Fig. S3).
The fluorescent label allowed the neural circuits to be visualized under a fluorescence microscope.
The edges of the defect could not be visualized under a microscope due to bone overhanging or a curved or narrow EAC in 8 anterior perforations.
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After 30 min, cells were briefly fixed with 4% PFA in PBS and slides were mounted using Profade Gold antifade reagent (Invitrogen) to be visualized under an inverted microscope.
The slides were visualized under a microscope.
Finally, the cells were visualized under a fluorescence microscope in 10 randomly selected fields for each group.
The haemotoxylin and eosin (H and E) stained sections were visualized under a light microscope (Nikon eclipse 50i, Japan) under 20X.
The intracellular localization of nanoparticles was visualized under a laser scanning confocal microscope (Bio-Rad MRC 1024, Tokyo, Japan) equipped with Argon (488 nm) and HeNe (543 nm) lasers.
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