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To confirm that m909 can indeed bind to FRβ on intact cells, we investigated whether m909 binding could be visualized on cell surfaces.
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Despite their low abundance in the carboxysome, the CsoS4 proteins could easily be visualized on an immunoblot of wild type cell extract probed with anti-CsoS4B antibody (Figure 6A, lane 3).
The epithelial-mesenchymal interface within the renal stem/progenitor cell niche can be visualized on a Richardson-labeled semithin section made from the outer cortex of the neonatal kidney.
In our study, mice showing blocked lymph drainage did not yet contain metastases in the ING or AX LNs, even though melanotic metastases consisting of less than 1,000 cells can readily be visualized on the LN cortical surface [ 23].
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The results were visualized on a Fluor chem system (Cell Biosciences).
Cell attachment was visualized on the scaffolds using toluidine staining and fluorescence microscopy, with cells appearing to attach well to the microspheres within the scaffolds and to spread over the scaffold structure.
Cells were visualized on a Leica DM LB microscope.
Cells were visualized on an Axiophot microscope (Carl Zeiss).
Cells were visualized on a Zeiss Axioskop microscope and images captured using a ZeissAxiocam camera.
Immunostained cells were visualized on an Olympus BX-51 epifluorescent microscope.
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