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For example, in 1980 Harris et al. demonstrated that cellular forces could be visualized by tracking the wrinkling formation of thin elastic silicone rubber substrates due to applied cell stresses [7].
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Traction forces applied by the cell to the elastic substrate caused substrate deformations that were visualized by tracking fluorescent marker beads in the uppermost layer of the substrate (see above).
In all these cases, LOH likelihood consequently decreased below statistical significance threshold and these events were missed to be visualized by CNAG in the HMM-LOH track, as illustrated in Figure 4 (see Additional File 3 for LOH likelihoods of the other deleted regions).
The genes for the detected ROIs can be visualized by the circular-graph, Circos, or as custom tracks in UCSC.
Peaks were visualized by preparing custom tracks using the UCSC Genome Brower (http://www.genome.ucsc.edu/) based on the protocol previously described [ 26].
Dynamic tracking behavior was visualized by adding medium strength ssrA degradation tags (C-terminal, RPAANDENYAAAV) to the scaffold red fluorescent protein (RFP) and antiscaffold yellow fluorescent protein (YFP) fusion proteins.
Immunofluorescence was visualized by sequential imaging, whereby the channel track was switched each frame to avoid cross-contamination between channels, using a Zeiss LSM510 Meta inverted laser scanning confocal microscope described here: http://nisms.stanford.edu/Equipment/LSM510Meta01v01.html.edu/Equipment/LSM510Meta01v01.html
The deformation of the closed polygonal grains is visualized by combining the above technique with a microstructural tracking technique, in which the paths of particles are reconstructed by matching each pair of particles in consecutive images.
Morphology was visualized by scanning electron microscopy.
Both particles were visualized by TEM.
Polymer remnants were visualized by polarization microscopy.
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