Cell-bound cIgE was visualized by flow cytometry with PE-conjugated hapten NP. sFcεRI efficiently blocked binding of cIgE to FcεRI expressed at the cell surface (Figure 4A).
To correlate the efficiency of siRNA uptake directly with the efficiency of GAPDH-specific knockdown, transfected GAPDH-specific siRNA was visualized by flow cytometry via a fluorescent Cy3 label (Fig. 5B).
After three days of culture, T cell proliferation was visualized by flow cytometry.
After washing, the label incorporated at the damaged sites of the DNA was visualized by flow cytometry using the Epics XL FACS and EXPO 32 software.
Ox40-cre-dependent loss of p56Lck expression could be visualized by intracellular flow cytometry using the 1F6 p56Lck-specific monoclonal antibody (Figure 2).
Similar to the TE26 reporter, Cre mediated recombination will allow for EYFP expression which can be visualized by fluorescence microscopy and quantified by flow cytometric analysis.
The blood flow changes can be visualized by comparing some representative blood vessels, such as the ones pointed by arrows V1, V2 and V3 in Figs. 2 (B) and (D).
Cells that expel the dye are visualized by dual-wavelength flow cytometry (FACS) as a 'Hoechst low' tail of cells, the SP, relative to a larger bulk of 'Hoechst high' cells, the main population (MP).
ZsGreen1 GFP expression in BMSC delivered by gene carriers was visualized by fluorescence and quantified by flow cytometry (B).
Forty-eight hous after transfection, fluorescence intensity was visualized by fluorescence microscopy, followed by flow cytometry analysis.
