Protein binding was visualized by detecting HRP with the OPD reagent (Pierce, Rockford, IL, USA) that resulted in a colored product.
This is most likely due to glycosylation because immunoprecipitated Slp1-3xFLAG can be visualized by Pro-Q Emerald stain, which detects periodate-oxidized carbohydrates.
The genes for the detected ROIs can be visualized by the circular-graph, Circos, or as custom tracks in UCSC.
Immunoreactive bands were visualized by ECL and detected by ChemiDoc MP Imaging System (Hercules, CA, USA).
Immunoreactive proteins were visualized by enhanced chemiluminescence detecting system.
Signals were visualized by enhanced chemiluminescence and detected in a Fujifilm LAS 4000 imaging system (Fujifilm Corporation, Akasaka, Japan).
After 1, 3, and 7 days culture, cell morphology observation was performed by fixing specimens in 4% formalin solution for 15 min and 1% Triton X-100 in PBS for 10 min. The cytoskeletons were visualized by actin (F-actin was detected using TRITC-conjugated Phalloidin; Millipore), and cell nuclei were visualized by DAPI (Beyotime Institute of Biotechnology, China).
The detected signals were visualized by an enhanced chemiluminescence reaction system, as recommended by the manufacturer (ECL-Plus, Amersham, Piscataway, NJ).
Membranes were further incubated with peroxidase conjugated anti-mouse or anti-rabbit antibodies and the detected bands were visualized by chemiluminescence reaction exposed to X-ray films.
Proteins were visualized by Li-cor Odyssey equipment, which detects near-infrared fluorescence.
