The membranes were developed using the SuperSignal West Femto Chemiluminescent Substrate (Pierce , Thermo Scientific Pittsburgh, PA, USA) and were visualized and quantitated with the Bio Spectrum 500 Imaging System (UVP, Cambridge, UK).
The actin membranes were also detected using the SuperSignal West Femto Chemiluminescent Substrate (Pierce , Thermo Scientific Pittsburgh, PA, USA) and were visualized and quantitated with the Bio Spectrum 500 Imaging System (UVP, Cambridge, UK) and relative amounts of PLK-1, MDM2 and p53 protein are reported relative to β-Actin.
The antigen-antibody complex was visualized and quantitated using an Odyssey system (LI-COR, Lincoln, NE, USA).
ECL reactions were performed using an Immun-Star WesternC kit (Bio-Rad) and proteins were visualized and quantitated with a FluorChem HD2 imager and AlphaEase FC software (AlphaInnotech).
Blots were visualized and quantitated by using a Li-Cor Odyssey Infrared Imager (Li-COR Biosciences, Lincoln, NE). 25 nM of siRNA specific for MDC1 or a control siRNA was transfected with Lipofectamine 2000 (Invitrogen, Carlsbad, CA), according to supplier's instructions.
The DNA bands were visualized and quantitated using a Phosphorimager.
The membranes were exposed to film and signals were visualized and quantitated using GelPro software.
Results were visualized and quantitated using a FUJIFILM-FLA-5000 FUJIFILM-FLA-5000 FUJIFILM-FLA-5000Ltd, Stamford, CT, USA).
Bands were visualized and quantitated using a digital image analysis system (Kodak 1-D Eastman Kodak Co, MA, USA).
The dried gels were visualized, and radioactive bands were quantitated using Phosphor Imager (Molecular Dynamics, Sunnyvale, CA) using Image Quant software.
Tris-Acetate 3 8% gels were run, Western blotted, visualized and quantitated according to manufacturer's instructions for the NuPage system (Invitrogen) and Odyssey Infrared imaging system (Licor Biosciences).
