To date, this rumor hasn't been verified by either Yahoo or Actionality.
Knockdown efficiencies were verified by either immunoblotting or quantitative RT-PCR (RT-qPCR).
Except for two interactions (TPK1 with GRF2 and GRF4), all other interactions were verified by either pull-down analysis based on GST fusions (interaction of TPK1 1-79 TPK1 1-791 and GRF6) [29] or Bimolecular Fluorescence Complementation (BiFC) experiments (interaction of TPK1(1-79) with GRF1, 5 and 6) [33].
The efficiency of gene knock-down by shRNA was verified by either immunofluorescence or immunoblot analyses.
Stable incorporation of the genes was verified by either Southern blot hybridization or PCR analysis.
Disease recurrence was verified by either imaging (PET/CT) or biopsy.
PCR product integrity was verified by either direct DNA sequencing, or ligating into pGEM-Teasy vector (Promega) followed by sequencing.
(2) Intra-abdominal abscess was verified by either surgical drainage or by ultrasonographically guided aspiration of pus.
Almost three-quarters (1582) of these FNBs were verified by either histopathologic diagnosis following surgical resection or clinical follow-up.
Specificity of staining was verified by either replacing the primary antibody with normal serum or preabsorbing the antibody with its corresponding immunizing peptide (sc-52P for c- fos, Santa Cruz Biotechnology, at 1 μg peptide per 1 μl anti-serum; AG344 for NR1; AG305 for GluR2/3, Chemicon, USA, at 1 μg peptide per 5 μl anti-serum).
