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In practice, such models do not always generalize to other locations, hence, they should be validated using independent data.
Therefore, our results should be validated using independent and bigger samples.
Furthermore, such candidate signatures must be validated using independent cohorts statistically powered to confirm the existence of a predictive signature.
The established infrastructure will enable SCAN-B-derived biomarker tosts to be validated using independent series of population-based cases from the ongoing prospective SCAN-B study.
Although our in silico data for most analyzed genes were either validated using qPCR or coherent with previous published data, gene expression should be validated using independent and sensitive methods, such as qPCR, before functional characterization steps.
While this remains to be validated using independent data, the receiver operating characteristic curve gives some insight as to the likely performance of the model using a predefined cutoff.
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Estimated AGB was validated using independent field measurements (R2=0.67, p<0.001).
The growth model was validated using independent data collected from another research area of the same forest type.
The calibrated models were applied to create prediction maps of SOC stocks that were validated using independent testing data.
A recent review by Schmokle et al. [25] reported that even today only 3% of models are validated using independent data.
Training of the model was achieved using a large amount of experimental data obtained from the H2-producing UASB reactor, whereas it was validated using independent sets of performance data obtained from another H2-producing UASB reactor.
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