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The NR model can then be validated using a smaller subjective test.
The proposed implementation model will be based on object-oriented techniques of simulation and will be validated using a pedagogical example.
Promising candidates can then be validated using a method with higher accuracy and proven reliability, such as ELISA.
Therefore, in a future work this approach should be validated using a larger and more generalizable dataset like the data provided by the Alzheimer's disease Neuroimaging Initiative (ADNI: www.adni-info.org).
In any case, to further evaluate the therapeutic potential of natural products identified using embryonic or larval zebrafish models, bioactive compounds should subsequently be validated using a series of additional in vitro and in vivo assays.
This strategy will need to be validated using a prospective protocol.
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Therefore, these identified subtype-specific metabolites can be validated using an independent dataset.
The model in each condition was validated using a leave-one-out cross-validation routine.
The discriminatory 28-gene profile was validated using a leave-one-out cross-validation test.
The results were validated using a standard leave-one-out cross-validation approach.
The quality of estimation is validated using a quadratic criterion.
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