Scripts, topics, and timeframes that were initially devised by content experts were validated further and revised for direct use or adaptation by clinicians in the conduct of focus group sessions for parents/guardians, siblings, and affected children.
These data should be validated further in larger prospective studies.
The specificity of the antibody directed against ATF7IP and the specific targeting of Atf7ip by the siRNA were validated further by immunostaining and knockdown, respectively, of ectopically expressed FLAG-tagged ATF7IP.
Of course, the results of our study should be validated and further refined in other large, independent and well-characterised cohorts.
New molecular techniques, especially PCR assays, still need to be validated and further developed in different settings.
These miRNAs and their target genes, reported in advanced stage RB, needs to be validated and, further, to be used either as biomarkers and/or for targeted therapy.
However, the utility of BBB EMVs as a source of disease-specific biomarkers remains to be validated in further in vitro and in vivo studies.
The prediction rules need to be validated externally and further research is needed to investigate the impact of vaccination on serotype replacement.
However, this hypothetical model needs to be validated by further identification of expressed and silent variant protein genes in other B. recurrentis isolates.
Monitoring indicators need to be validated, and additional ones further developed as a priority area for research.
