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We note that these findings need to be validated by additional inspection.
However, the concepts demonstrated by this kinetic approach will need to be validated by additional prospective clinical studies.
By using physicochemical predictions or three-dimensional data, the nature of interaction can be evaluated in silico, but remains to be validated by additional biological experiments.
In this case, generally well performing methods should be applied and, if possible, results should be validated by additional experiments (e.g. RT-PCR).
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Furthermore, this structure has been validated by additional docking studies.
The accuracy of the proposed equation was validated by additional five groups of BFRP tubes with different slenderness ratios.
Our initial findings from Han Chinese have since been validated by additional samples from the Chinese [6], Portuguese [7], German [7] [9] and Japanese [9] populations, although conflicting results from Japanese [10] and German [11] populations have also been reported.
These results were validated by additional gene expression analysis performed in-house.
Visual inspection of the networks was validated by additional analysis with alternate preprocessing and statistical comparisons of patient subsets.
The proposed modes of regulation were validated by additional microarray experiments in which the male and female isoforms of DSX were over-expressed (data not shown).
Some of these studies were validated by additional computational methods while others used RT-PCR or Northern blotting experiments to confirm the initial database searches.
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