Make sure you are using a beer that is meant for a chilled glass.
To extract antioxidative enzymes, fresh leaf samples (1 g) were homogenized using a chilled mortar and pestle in 5 ml of Na-phosphate buffer (100 mM, pH 7.8) containing EDTA (1 mM), dithiothreitol, (DTT, 1 mM) and polyvinylpyrrolidone (PVP, 2% w/v).
Images were captured using a chilled, charge-coupled device camera (Hamamatsu, Bridgewater, NJ) and SmartCapture software (Digital Scientific, Cambridge, UK).
Kidney tissue (∼100 mg) was pulverized using a chilled mortar and pestle and then resuspended in 3 mL modified radioimmunoprecipitation assay buffer containing 50 mM Tris HCl (pH 7.4), 1% NP-40, 0.25% Na deoxycholate, 150 mM NaCl, 1 mM EDTA, 1 mM phenylmethanesulphonyl fluoride, 1 µg·mL−1 aprotinin, 1 µg·mleupeptinptin, 1 µg·mL−1 pepstatin, 1 mM Na3VO4, and 1 mM NaF.
Air defrosting can be used only in chilled chambers at temperatures above zero, 2°C or higher.
To the remainder, one-sixth volume of buffer A containing 0.2M sucrose and 1.2% Triton N-101 was added and the samples were homogenized (30 strokes using a chilled dounce with a 0.004-0.006 inclearancence pestle).
In recent years, the term "chillax" has come to be used as a hybrid of chilled and relaxed, although it is most likely to be used by people unfamiliar with either state.
Insect bodies were crushed in liquid nitrogen using a chilled mortar and pestle and an extraction buffer (0.2 M phosphate buffer pH 7.6 containing 2 mM phenylmethanesulfonylfluoride) was added at a ratio of 3 mL buffer per gram of insect powder.
About 0.50 g of frozen leaves were ground to a powder using a chilled mortar and pestle with liquid nitrogen and a small amount of insoluble polyvinylpolypyrrolidone, then homogenized with 3.5 mL cooled extraction buffer containing 50 mM K-phosphate (pH 7.5), 1 mM EDTA, 10 mM MgCl2, 1 mM ascorbic acid, 12%% (v/v) glycerol and 0.1%% (v/v) β-mercaptoethanol.
Samples, 0.1 g for each sample, were homogenized in liquid nitrogen using a chilled mortar and pestle, with 50 ml of buffer containing 50 mM Tris HCl pH 7.2, 50 mM trans-1,2-diaminocyclohexane-N,N,-tetra-acetic acid (CDTA) and 25 mM dithiothreitol.
