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Alternatively, NGS sequencers can be used to sequence targeted genomic regions from a very large number of samples arranged in multidimensional pools.
Unlike real-time RT-PCR assays, the conventional type-specific primers can also be used to sequence any of the cDNA amplicons that are generated, providing further and definitive proof of the relationships between individual virus strains in Seg-2.
UniNovo also can be used to sequence paired spectra (e.g. CID/ETD spectral pairs).
Alternatively, the primer can be used to sequence the transposon junction directly [ 6].
Alternatively, it can be used to sequence 1 Mb of the human genome to 6000× coverage.
These technologies could, however, be used to sequence extremely large SAGE libraries.
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Morpholino antisense oligonucleotides can be used to sequence-specifically block translation [ 10] or pre-mRNA splicing [ 11].
Cationic pyrimidine bis-PNAs can be used to sequence-specifically bind to one strand of the DNA duplex, leaving the other strand free for probe hybridization.
With this development came established methods and analytic standards that were used to sequence other large genomes.
This method was used to sequence the NCRs of A/Bilthoven/16190/68 (H3N2) and A/Turkey/Turkey/1/05 (H5N1).
The contour difference detection operation is used to sequence the points for each layer.
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