Density gradient-based isolation using sucrose or iodixanol (OptiPrep™) can be used to isolate each EV fraction with greater purity than other methods.
Ion exchange can also be used to isolate the analyte.
We hypothesised that the TEO trapping methodology could be used to isolate previously unidentified CO2-binding sites on proteins.
It can be used to isolate and control the sharpness, tone, and contrast of a photograph at various scales.
The company said the process could be used to isolate either version of a molecule and that it could be applied to thousands of amine compounds.
Our work has shown that this receptor plays a role in the formation of cancer stem cells and can be used to isolate and target this cell population.
MARIS can therefore be used to isolate high quality RNA from many otherwise inaccessible cell types simply based on immunofluorescent tagging of unique intracellular proteins.
Counterbalanced latin squares, which have every treatment preceding every other treatment the same number of times, can be used to isolate treatment effects from carryover effects.
A similar approach can also be used to isolate large quantities of inner hair cells and selected supporting cells from the whole-mount cochlear preparation.
The first modification we describe, a ChIP cloning strategy, can be used to isolate any genomic fragment specifically associated with a particular factor.
I recommend experimental designs that could be used to isolate the role of the different mediators and suggest the incorporation of process measures.
