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This type of analysis can greatly contribute towards understanding the function of newly annotated genes since it can be used to functionally associate them with genes that have well-defined functions in specific biological processes.
MapMan is a useful tool to provide global views of diverse aspects of high-throughput data and can be used to functionally classify rice genes with flanking sequence tag (FST) mutants for systematic functional studies (Thimm et al. 2004; Usadel et al. 2009; Jung and An 2012).
Thus quantitative far-Western blotting appears to provide additional information on tyrosine kinase signaling state that can be used to functionally classify cells on the basis of RTK activation status.
Taken together these results demonstrate that QDs can be used to functionally label native proteins and show that detectable pull-down assays can be performed on a single affinity bead.
Importantly, the same cell lines used for discovery can also be used to functionally examine cancer gene candidates, for example using RNA interference to knockdown the expression of amplified oncogene candidates, and then assaying loss of tumorigenic phenotypes in cultured cells or in vivo (e.g. refs.[90], [91]).
This approach will be used to functionally estimate the contribution of miRNA dysregulation to tumorigenic potential.
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Three databases of Arabidopsis gene sets involved in the gene ontologies of biological processes, cellular components, and molecular functions were used to functionally annotate the metagenes.
The Altera Stratix III FPGA kit is used to functionally verify the proposed design while Synopsys Design Compiler is used to estimate the chip area and the design static power consumption (ASIC design).
Two-electrode voltage clamp electrophysiology was used to functionally characterize candidate receptors expressed in Xenopus oocytes.
The Gene Ontology Database (http://www.geneontology.org/) was used to functionally categorize gene expression profiles.
Ingenuity Pathway Analysis (Ingenuity Systems, http://www.ingenuity.com/) was used to functionally annotate genes according to biological processes and canonical pathways and to search for potential biomarkers.
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