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Players can carry an unlimited number of weapons, and certain guns can be used in duplicate, one in each hand.
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Five different inclusion levels of hyp were used in duplicate tanks; no addition, 0.7, 1.4, 2.8 and 5.6 g kg− 1.
All sequencing primers listed in Table S1 were used in duplicate or quadruplicate for each atypical BSE sample to obtain multiple genotypes of each htSNP.
Three controls with related RNAi cell cultures were used in duplicate on three different cards and the data analyzed together as a relative quantity (RQ) study.
The blood was immediately centrifuged at 13,000×g for 3 mins at room temperature and 50 µL of plasma was used in duplicate to perform indirect ATP measurements using the CellTiter-Glo Assay (Promega) according to manufacture's instructions.
The Quantigene plex assay was used in duplicate to determine GAPDH mRNA expression.
This setup was used in duplicate for each experiment, and the data obtained from each set of cages were combined.
A total of 10 μl of lysate was used in duplicate to quantify protein content for normalization.
Serial dilutions of the stocks were carried out in duplicate, and dilutions in the range from 10-108 copies were used in duplicate PCR to generate standard curves.
Slope values were calculated using software from Applied Biosystems (SDS1.2) and an input of 50, 5, 0.5, and 0.05 ng (102 – 10-2) DNA was used in duplicate.
Primer sets for CCL9, Msr1, CCL17, and MMP12 were used in duplicate assays and β-actin RNA was used as an internal control.
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