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As it gets narrowed down, overlapping peptides could be used in binding assays to define the boundaries.
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Synthetic peptides were used in binding, aggregation and precipitation studies.
HT-29, Ramos and CEM were used in binding assays by flow cytometry as described above.
These cell lines were used in binding assays as described above.
rRH5 was used in binding experiments with freshly prepared resealed red cell ghosts (to avoid fluorescence quenching by hemoglobin).
For super-shift of protein-DNA complexes, 2 µg of respective antibodies were used in binding reaction.
Lysates from MDMs were used in binding reactions with GST-HIV-1 Nef constructs.
Confluent monolayers of 293 + αvβ3 cells were used in binding assays.
Next, immobilized GST-PWWP was used in binding to mono-nucleosomes prepared from mammalian cells.
Lysates from bacteria expressing N-terminal HA-tagged eEF1A mutants were used in binding reactions with GST-HIV-1 Nef.
To test this possibility, a FtsZ truncation mutant, FtsZ(1 360), was used in binding assays with SlmA DNA.
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