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Both the EpiTect Methylight and QuantiTect Multiplex Mix buffer systems could be used for multiplex qMSP.
Markers that yield positive signals in one plate can be used for multiplex PCR.
This approach appears feasible for individual miRNA marker quantification but cannot be used for multiplex miRNA evaluation.
The chip could also be used for multiplex gene detection when combined with spectrally resolved probes in each reaction chamber.
Based on this result, Chen, et al. suggested that stem-loop RT primers can be used for multiplex RT reaction and small RNA cloning for better efficiency and specificity [ 22, 24].
In addition to being used for GWS, DBSS can also be used for multiplex protein measurements [ 21], quantitative RNA micro arrays detecting up to 3000 genes [ 24], and quantitative DNA methylation analysis [ 25].
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A biotinylated consensus primer, GP6+, and 15 high-risk HPV type-specific primers are used for multiplex PCR.
PrimerPlex 2.0 (Premier Biosoft International) was used for multiplex PCR signature design.
A compatible set of 96 barcode sequences that have been used for multiplex sequencing is provided as supporting information (Table S1).
Two primer sets (PCR1: bbp, cna, ebpS and eno; PCR2: fnbA, fnbB, fib, clfA and clfB) were used for multiplex PCR.
In a recent study by Ingvarsson et al, 2007, sandwich antibody microarrays were used for multiplex analysis of eight complement proteins (including C3) in unfractioned serum and plasma [23], showing promising results for protein profiling in complex samples.
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