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This is primarily in order to obtain the volumetric scaling factor, to be used as normalization for head size.
The brain image is then affine-registered to MNI152 space (using the skull image to determine the registration scaling) [27] [28] in order to obtain the volumetric scaling factor to be used as normalization for head size.
Although 18S and 28S % areas could be still be used as normalization markers for FMR1ex3.4 and FMR1ex13.14, the most optimal of the predictors tested for both DNMT1 and FMR1 was GUS.
Therefore, the two Rluc-encoding vectors reported here cannot be used as normalization controls in all transfection systems because of their inducibility by experimental treatment and potential misinterpretations of reporter gene activity.
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Beta-actin was used as normalization control.
β-Actin primers were used as normalization control.
To analysis the expression of CsPI in wild-type and pi-1 mutant A. thaliana, the A. thaliana housekeeping gene ACTIN was used as normalization control.
GAPDH amplifications were used as normalization controls.
Glucuronidase primers were used as normalization control.
The latter two cassettes were used as normalization controls for each experiments.
Untreated cells were used as normalization control.
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