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Proteins that are stably palmitoylated, such as caveolins (Parat and Fox, 2001), can be used as loading controls in the assay (Tulloch et al., 2011).
For each set of experiments, two gels were run in parallel; one of them was immunoblotted and probed with an antibody against Gus (Fig. 9a, d), and the other gel was stained with Coomassie blue to be used as loading control (Fig. 9b, e).
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The known amount of loaded protein was used as loading control.
GAPDH levels were used as loading control.
Tubulin was used as loading controls.
H3 and GAPDH were used as loading controls.
Anti-β-actin antibody was used as loading control.
Tubulin was used as loading control.
Actin and ponceau were used as loading controls.
Actin, tubulin and cytochrome-C were used as loading controls.
The GAPDH signal was used as loading control (Santa Cruz Biotecnology, Santa Cruz, CA).
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