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The approach eases jet lag, allows time for acclimation and is a good way to scope out some areas that may be unique sites for business meetings or to visit on personal time.
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There are unique sites for RsaI and HpaII/ MspI within the IGS tandem repeat, and its cleavage with the indicated restriction enzymes should result in the formation of 225 bp DNA fragments and thus correspond to the experimentally observed data.
IPMCs are constitutive features of MDMs that are continuous with the plasma membrane and are used as unique sites for the assembly of new virions following infection by HIV-1.
Collectively, these experiments show that DRIL1 is sumoylated in vivo and that lysine 398 is the unique site for this modification.
A password should be complex, but memorable and should be unique for each different site or service.
Ideal cloning strategies contain only unique restriction sites (only present once) to ensure that cloning is directional and straightforward, hence requiring two unique sites for each insertion event.
Iron is essential for vital redox activities in the cell, in particular it is required for respiration and energy production in mitochondria (which are also the unique site for heme synthesis and the major site for Fe-S cluster biosynthesis), and likewise is important for mitochondria biogenesis.
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Table 4 lists the restriction enzyme recognition sites found in the MCS for each plasmid and indicates which sites are unique for each construct.
In short, a total of 49 QTL was detected and when the two field sites are combined, eleven QTL were found at both sites, whereas 27 QTL were unique for one of the sites.
A blast search for each primer pair verified that the binding sites were unique for each gene studied.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com