Expression of tGLI1 is undetectable in normal cells but is high in glioblastoma multiforme (GBM) and other cancer cells.
PLIN1 is undetectable in normal liver, but is expressed in the liver of humans with NAFLD (Straub et al., 2008).
Snail1 RNA is undetectable in normal colon mucosa but it becomes upregulated in 60 70% of colon cancers [10] [12].
It has previously been shown that YB-1 is undetectable in normal breast tissue [39] or in normal bone marrow plasma cells [40].
However, the most prominent structural alteration was found in the lowest Stx1 positive band of the cancerous tissue by detecting [M5*+Na]+ ions (T, right panel), which were undetectable in normal tissue (N, right panel).
For example, a recent immunohistochemistry (IHC) study indicated that the GHSR 1b isoform is undetectable in normal breast tissue, but dramatically up-regulated in all breast tumors analyzed [33].
Here, we found that expression levels of the BH3 only proteins Bax and Bak were undetectable in normal human hepatocytes, while the HCC cells showed clear high expression of these proteins.
In comparison with HCC cells, levels of pro-apoptotic Bax and Bak were undetectable in normal human hepatocytes, anti-apoptotic Bcl-xL levels were similar in all the cell lines tested, and Bid expression levels were much higher in HCC cells than those in normal human hepatocytes.
Recently, we have demonstrated that hMena overexpression represents an early marker of breast tumorigenesis, being undetectable in normal breast epithelium, and overexpressed in benign breast lesions with an increased risk of transformation and, in more than 70% of tumors, with HER2+, ER/PgR- and high Ki67 phenotype [15].
