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The gene sections corresponding to the 5' and 3' untranslated regions (5'UTRs and 3'UTRs, respectively) are also likely to be under less selective constraints than the ORFs, yet signatures of strong selection in UTRs have been described (reviewed in [ 5]).
The rationale for choosing 4D SNPs is that they do not cause amino-acid changes and thus, should be under less selective pressure and more reliably reflect population structure and demography18.
Under these conditions the bacteria grow slowly, have less competition, and therefore may be under less selective pressure to maintain gene order.
This supports the conclusion that both genes could be under less selective pressure in Zambia.
Since the dN/dS values of HI genes were greater than the housekeeping genes, HI genes might be under less selective constraints than housekeeping genes.
Following a duplication event one of the two (paralog) genes might keep its original function, whereas the other one might be under less selective pressure.
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The high accumulation rate of mutations can be interpreted partially as genes being under less selective constraint.
First, we observed that maternal genes were under less selective constraints while there were strong selective constraints effecting on earlier zygote-activated genes.
However, editing sites that are temperature-sensitive are also more variable across species, suggesting that labile RNA structures are under less selective pressure.
The higher polymorphic potential of ILP markers is expected because of their development targeting the hypervariable introns, which are under less selective pressure.
The CVRE hypothesis is also consistent with the fact that a recessive variant is more likely to rise to a common allele frequency than a dominant or additive variant because it is under less selective pressure (Curtis 2013).
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