The mutants had a wide range of effects on propagation of [ PSI+], with some being unable to propagate the prion at all (G41D, G50D, D236N, G342D, E370K, and G616D) to others having minor effects on color phenotype (P37L, C211Y; Table 3 and Figure 1B).
Whereas Sp_Hsp104 is unable to propagate the [PSI+] in S. cerevisiae, does it assist in the propagation of prions in S. pombe?
It was unable to propagate itself institutionally into the next generation.
By reducing the holes to six in each ring and pulling the fibre to appropriate dimensions, light at 633 nm leaks out between the holes and is unable to propagate along the fibre.
However, unlike Sc_Hsp104, the S. pombe homolog is unable to propagate [PSI+] in Δhsp104 cells or to cure this prion by overexpression.
Incompatibility tests were done as previously described [19], and confirmed that the pX1.0 plasmid was unable to propagate in the presence of the IncX1 plasmid, pOLA52.
This demonstrates that Sp_Hsp104 is unable to propagate [PSI+] and/or that interaction with species-specific cofactors are required for this activity.
Conversely, an unfit strain with a high seeding rate is unable to propagate, effectively 'immunising' the population and thus further delaying the wild type outbreak associated with stockpile expiry.
However, the terminally differentiated oocytes are unable to propagate themselves.
