The washed mycelia were transferred to induction medium containing an appropriate carbon source and incubated for appropriate periods at 28 °C with shaking (220 rpm).
The recovered material with high Si content can be transferred to an induction furnace to generate solar-grade Si.
Interestingly, microvesicles content could be transferred to endothelial cells, resulting in the induction of insulin mRNA expression and protection from apoptosis and angiogenesis.
After cocultivation, the explants were transferred to callus induction medium (MSB containing 0.1 mg/L 2,4-D, 0.1 mg/L KT, 0.1 mg/L IAA, 30 g/L glucose, 2 g/L Gelrite, pH 5.8) supplemented with 500 mg/L cefotaxime and 50 mg/L hygromycin.
Each transformant was pregrown on glucose and mycelia were transferred to an induction solution containing α-sophorose, cellobiose, or Avicel as an inducer.
After 3 days of co-cultivation at 22°C in the dark, segments were transferred to callus induction and selection medium (same composition as the co-cultivation medium, but with no acetosyringone, and containing 200 mg/L vancomycin and 25 mg/L kanamycin).
Reaching 100% confluence, cultured periosteal cells were transferred to the osteogenic induction medium.
Following bombardment, the immature embryos were transferred to the callus induction medium, supplemented with 50 mg l-1 hygromycin B (Sigma) and maintained in the dark at 27°C for 45 days.
The transformed hygromycin-resistant regenerated shoots about 2 to 3 mm were transferred to β-estradiol induction medium without hygromycin to induce marker excision.
(3) Food was provided when the eggs were transferred to the hatching induction conditions, and hatching was induced at 12 g L-1 salinity; in this way the hatchlings were already in the growing conditions when they were born.
