These data will then be transferred into the statistical package STATA version 10[ 21] for statistical analysis.
From the database, details can be transferred into an Excel file for statistical analysis.
All variables and changes will be transferred into the Satastical Package for Social Sciences (SPSS), SYSTAT™ statistical software, summarized (Counts, Minimum, Maximum, Mean, Median, Standard Deviation) within each treatment group, transferred to the statistical report and then reformatted.
The PCR products from the CLPA were analyzed on the CE instrument and the raw data from the CE were transferred into a statistical data spreadsheet file (e.g. Microsoft Excel) to provide the normalized data using the housekeeper (Figure 4).
In addition, the distance matrices based on the concatenated housekeeping gene, ospC and ospA sequences obtained with MEGA were transferred into Minitab Statistical Software® (Minitab Inc., State College PA, U.S.A). to generate histograms of the frequency of genetic distances in B. lusitaniae.
Values from each biological replicate were transferred into the statistical analysis program GraphPad (GraphPad Software, Inc., La Jolla, CA, USA), verified for normality, and an analysis of variance (ANOVA) run to assay differences between experimental treatments.
For quantitative analysis of DNA methylation, percentages of DNA 5-methylcytosine and 5-hydroxymethylcytosine derived from the formulas listed above were transferred into the statistical analysis package GraphPad, verified for normality, and an analysis of variance (ANOVA) run to assay differences between experimental treatments.
Using this map, the central slice of each delineated druse was selected and its characteristics were transferred into SPSS for further statistical analysis.
The fluorescence intensity levels are presented relative to baseline and shown as Δ F/ F. Data were transferred into MS-Excel program for further statistical analysis.
All data were transferred into SPSS 20.0 or SAS 9.2 for statistical analysis.
