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Cell aggregates containing about 20 000 cells, equaling 10 30 colonies, were transferred into cryo vials.
An equal volume of media were added to each well and cells were transferred to Eppendorf tubes and centrifuged at 1000 × g for 5 min.
At predetermined time intervals, gels were transferred to an equal volume of fresh PBS and the collected samples were stored at −80 °C until protein quantification.
Equal volumes of serum were transferred into two transfer tubes and stored at −20°C pending analysis.
Equal aliquots of separated plasma were transferred into two labelled cryovials and frozen at −70°C.
Linda found refuge at a national shelter before being transferred to 'Different and Equal', a UN Women-supported civil society organization that works with Albanian trafficking survivors.
After centrifugation at 12,000 g for 15 min at 4°C, the upper aqueous phase was transferred to new tubes containing an equal volume of 70% ethanol.
The equal amounts of cell lysates were transferred to 96-black well plates and the fluorescence was measured at λ = 485 nm excitation and λ = 538 nm emission.
The aqueous phase was transferred to a tube containing an equal volume of chloroform, mixed well and centrifuged at high speed.
The supernatant was transferred to a new tube and mixed with an equal volume of isopropanol followed by centrifugation at 12,000 g for 15 min at 4°C.
They were assisted in finding equal paying positions, or have been transferred to desk jobs.
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CEO of Professional Science Editing for Scientists @ prosciediting.com