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Monitoring infection efficiency with the GFP-expressing pCL-retroviral vector, revealed that the majority of rat NPs (>95%) could be transduced (data not shown).
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In contrast, presence of integrated vector could not be detected in any of NBT-negative clones derived from transduced EBs, indicating that they were not actually transduced (data not shown).
Together, our data provide evidence that Wnt-mediated axon guidance can be transduced through a β-catenin-dependent pathway.
FACS analysis showed that over 90% of HeLa cells were transduced by a EGFP-expressing adenovirus (data not shown).
The mechanism by which this information is transduced to the cell is currently not well understood.
Each virus also encodes the green fluorescent protein (GFP) for the microscopic monitoring of transduction efficiency and ∼95% of the Schwann cells were transduced, as visualized by the presence of the green fluorescence (data not shown and Figure 1).
One cohort of BEAS-2b cells were transduced with HD-Adv and other cohort was not transduced and cultured for 24 h.
These data showed that CysC is transduced into the cells via the clathrin-dependent pathway and localized to lysosomes.
To improve its immunogenicity, the cell line was transduced with the costimulatory molecules CD80 (B7-1), which was efficiently expressed on the cell membrane (data not shown).
Our results showed, in addition, that a coding region of c-myb which is not essential for transformation was transduced by avian myeloblastosis virus.
MNs were transduced and, 8 days after plating, were treated or not with BafA1 (50 nM during 4 h).
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