Cultures that contain more than two distinct cell types (e.g., cancer, stromal, and endothelial cells) can be assembled in the same manner, provided that each cell can be traced using a distinct fluorescent reporter, or cell specific marker (e.g., CD31 for endothelial cells).
In this study, the post-buckling paths are traced using an algorithm that combines the arc-length iterative procedure with the modified Newton Raphson method.
The post-buckling equilibrium paths are traced using an arc-length solution scheme in conjunction with the modified Newton Raphson method.
Ductal cells were traced using an adenoviral reporter construct in which the CK19 promoter was used to drive GFP expression.
Axons were traced using an automated pipeline (Oberlaender et al. 2007; Oberlaender, Broser, et al. 2009).
Muscle section perimeters were traced using an ×4 objective and the location of all identified MS plotted using an ×10 objective for stained sections.
Lateral cephalograms were traced using a 0.03-in.
To measure the NF, the central non-enhanced area was traced using a dedicated contour (d, h).
The core was traced using a known concentration of deuterium oxide.
The postbuckling path is traced using a combination of the arc-length and mesh-free kp-Ritz methods.
