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The chessboard showed that a dilution of 1.5625 μg/ml of 2F10 and 0.0975 μg/ml of 1F11b would be the optimal concentrations for the sandwich ELISA.
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Studies in vitro revealed that cell proliferation, ALP activity and mineralization were induced by hPRP and 10 12.5% of hPRP seemed to be the optimal concentration.
Based on the presented results, 50%% HMTA appears to be the optimal concentration, as it yields a high density of nanorods with good uniformity.
Mn2+ at 1.5 mM was determined to be the optimal concentration for the strain comparison.
We found 25 nM to be the optimal concentration of each siRNA for individual gene products.
Thus, 10−7 m 17β-oestradiol was selected to be the optimal concentration and was used for the following experiments.
Loetscher and colleagues report a dose-responsive neuroprotective effect, with 50% argon appearing to be the optimal concentration for neuroprotection.
These concentrations may not be the optimal concentration for the local delivery of ZVAD and Nec-1 to the middle ear of the adult mice.
This was found to be the optimal concentration from our previous studies [ 20] since higher concentrations were toxic while lower concentrations led to insufficient activation of DCs.
Immunoblotting showed that 0.3-1% Triton X-100 were the optimal concentrations for the extraction and purification.
We note that, the highest capacity, average discharged voltage and initial Coulombic efficiency were observed for 5 M KFSI electrolyte indicating that it is the optimal concentration among different KFSI molarities in EC/DMC (Supplementary Fig. 12).
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