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One reason for this could be the complex binding biology of these two protein molecules.
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Further increasing the weight ratio, the excessive cationic polymer contributed to the surface positive charge, which is necessary for the complexes binding to anionic cell surfaces for cellular internalization.
One important complex involved in regulation of the ALAS encoding gene is the CCAAT core binding complex (CBC).
Plx1 was unable to bind to chromatin in extracts depleted of the Mcm complex, suggesting that the Mcm complex is the major binding site for Plx1 on chromatin during S-phase.
Each compound was docked to the protein complex binding pocket, and a score from the docking was assigned to each compound according to the weighed component of the ICM scoring function (see below).
DOI: http://dx.doi.org/10.7554/eLife.01008.003 These observations raise a fundamental question: how is the binding of Arp2/3 complex to filament sides coordinated with binding and release of VCA and the initiation and growth of the daughter filament?
Mbp1 is the DNA binding component of MBF complex, which binds to the promoters of DNA synthesis genes and regulates gene expression during G1/S transition [ 11, 16- 18].
The absence of thiolase in this complex can be accounted for by two hypotheses; firstly PTS2 cargo may be unloaded from the complex through binding with PEX14N, or secondly PEX14N may show a higher affinity for PEX5 PEX7 than for PEX5 PEX7 PTS2.
The mixture was centrifuged to separate the protein complex binding to the beads (pellet) and unbound solution (supernatant).
Along similar lines, the localization of ATR to the break site and its subsequent activation is dependent upon the 9-1-1 complex, between between ATR and ATR-interacting protein (ATRIP), and replication protein A (RPA).
An additional part of the nuclear BCL-3 complex is the BCL-3 binding protein B3BP thought to be involved in DNA repair, which forms a complex with p300 and BCL-3 [ 42].
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