The plasmid p201N Cas9 gRNA is the binary vector used for hairy-root transformations.
These are the binary vectors and can be maintained in both E. coli and A. tumefaciens.
For a protein pair, let be the binary 'pathway vector' indicating the pathway membership of h.
These conflict results might be due to the binary vector pCAMBIA1391Z used in this transgenic study.
As this vector was derived from the binary vector pCAMBIA0380 (CAMBIA, Canberra, Australia), it is suitable for A. tumefaciens-based transformation into Z.
CrW was transformed with the binary vector pSKan-35SGtF3H, and 46 transgenic torenia plants were regenerated and cultivated until flowering.
The resulting plasmid was digested with BamHI and KpnI, and the TAGL1 cDNA was subcloned into the binary vector pROKII [75] to generate an overexpression (35S TAGL1) gene construct.
The absolute ranking is used to direct a step-wise analysis of the differential distribution of 1's as they are encountered in the binary vector.
For plant transformation, the Zera-ECFP, SPg-ECFP and SPg-ECFPKDEL protein-coding sequences were introduced into the binary plant vector pCambia 2300 and the Zera-Ct and Zera-EGF-coding DNAs were introduced into the binary vector pBin19.
The complete reporter fragment was inserted in the binary vector pGreenII017968 using KpnI/XhoI restriction sites.
