The replication and retention of small plasmids being entirely dependent on the integrity of oriP led to the assumption that oriP might be the only active origin in the context of the entire EBV genome.
We show that DS is the most active origin of replication present in the mini-EBV genome regardless of its location, and it is characterized by the binding of the origin recognition complex (ORC) allowing subsequent replication initiation.
The translocation experiments demonstrated by ChIP and initiation start site mapping that DS is the predominately active origin in context of mini-EBV genomes.
Preparations in which cardiotonic steroids of both vegetable and animal origin are the active principles have been used as emetics, diuretics, and arrow poisons for centuries.
Innate heat of a celestial origin is the active element that mediates between the state of utter immobility which characterizes intelligible substances and the incessant mobility that defines the life of material beings.
These clusters were named ori/rep sequences, and analogous to those detected in mammalian mitochondria, three to four of these elements (Lecrenier and Foury 2000) are thought to be active origins of mtDNA replication initiated via transcription mediated through the mitochondrial RNA polymerase, the Rpo41 protein in S. cerevisiae (Fig. 8A).
Furthermore, the active origins have been experimentally studied in three model systems: Halobacterium sp. strain NRC-1 (Berquist and Dassarma 2003; Coker et al. 2009), Haloferax volcanii (Norais et al. 2007; Hawkins et al. 2013), and H. hispanica (Wu et al. 2012, 2014).
Second, Rbf1N colocalizes at interbands with acetylated histone H4 (Figure 4), a histone modification that has been shown to be associated with active origins of replication in Drosophila amplification stage follicle cells [16], [34].
The scattered disc, however, is dynamically active, and is far more likely to be the place of origin for comets.
All peaks from T3 that exceed the noise threshold were considered as active origins in the presence of HU.
