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It might be that this method for monitoring NF-κB activation is just less sensitive than the reporter gene assay.
A possible cause for this could be that this method was designed to work with two separate "bipartite" ligation probes contrary to the PLP system which was used in this paper.
An interpretational difficulty here would be that this method measures fluorescein leakage, a non-functional molecule, which may not neccasarily be as physiological a measure as anterior chamber flare readings, which correlate with albumen and IgG leakage[ 1].
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The problem was that this method was unreliable.
Another advantage of SBM is that this method can remove sources arising from artefacts.
The problem is that this method favours the discovery of large planets close to their stars.
My guess is that this method evolved as a way to avoid overcooking, but as long as you are attentive, it should not be a problem.
Novel thing is that this method does not need any organic solvent as superhydrophobic layer.
The problem from Reddit's perspective, of course, is that this method of sleuthing would be far less exciting for users, and would probably generate less traffic, than its current free-for-all approach.
The first is that this method changes the computational grid in order to maintain a uniform approximation.
In other words, Apple's intention is that this method should eventually become the standard.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com