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In spite of the difficulties with interpreting gene tree conflict as a specific sequence of evolutionary events, such patterns can certainly highlight the importance of such events, pinpoint the taxa involved, and yield hypotheses to be tested with targeted sampling within and among conflicting taxa, and with data from additional nuclear loci.
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All these applications need further development and are yet to be tested with the target users.
While these alternative MAGs are expensive, the fact that they are more generally available means they will be tested with diverse targets.
This strategy has yet to be tested with siRNA against HIV-1 targets, and in vivo electroporation will need to target a wider range of locations that include other regions of high T-cell concentration, such as the spleen and blood.
Genomic DNAs from a collection of 31 B. mallei and 34 B. pseudomallei isolates, obtained from various geographic, clinical, and environmental sources over a 70-year period, were tested with PCR primers targeted for each of the B. mallei ATCC 23344-specific nucleotide sequences.
The docking of a compound collection is tested with the target structure and a quantified interaction score is used to identify candidate lead compounds.
The vectors were tested with 20 target proteins and the results suggest that the vectors are useful both as a rescue strategy if the N-terminal hexa-histidine tagged construct does not express and also as part of a primary expression experiment.
The effect of various reducing agents on in vitro protein ligation was tested with several target proteins.
Finally, the accuracy of our method in predicting the FDRs was compared to that of the common approach where an equal number of decoy glycopeptides were tested with the target glycopeptides.
Two combinations of PINK1 siRNA were tested, with each siRNA targeting a different region of PINK1 mRNA.
The serum samples were tested with a competitive ELISA targeting influenza A nucleoprotein antibodies following the manufacturer's instructions (ID Screen, Influenza A NP Antibody Competition, ID.VET).
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