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This should be tested further using nuclear genetic markers, and if hybrids are identified, then this could be followed by experiments on hybrid fitness.
In addition to improving the performance of TopSort, its assignments could also be tested further using alignment to transcriptome or genome sequence from a clonal, axenic Symbiodinium strain (preferably of clade A), essentially as we have already done using Aiptasia genome sequence.
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The GF algorithm is tested further using experimental data acquired from the simple optical setup shown in Fig. 7.
These colonies were tested further using BiOLOG identification system to confirm their identity as E. coli and for any isolate identified as E. coli O157 H7 additional confirmation by serology may be recommended for the reasons of serious implications when this pathogen is determined in a food sample.
The extent of congruence was tested further using the randomized test [66], [68] by comparing the log likelihood for the 7 individual locus ML tree topologies with equivalent values for 200 randomly generated trees of the same size for each gene.
The 'ineffective' drugs (IC50 value >10 μM) were tested further using clonogenic assays.
These candidates were tested further using Northern blots, showing that differences in expression were primarily due to developmental stage rather than colour pattern.
This was tested further using an Uba80 expression construct created by placing Uba80 under the control of a tetracycline (tet -repressible promotet -repressible
Clostridium-suspected colonies were tested further using PCR assays for C. difficile-specific GluD targeting the GDH gene [ 15], C. difficile ribotyping [ 16, 17], enterotoxin A (tcdA) [ 5], cytotoxin B (tcdB) [ 18] and 16S rRNA PCR [ 19].
To exclude false positives, the 519 colonies were tested further using a multipoint tetracycline resistance assay (Fig. 2) where growth was measured in the presence of a range of tetracycline concentrations (0 μM, 5 μM, 9 μM, 18 μM, 36 μM, 72 μM and 144 μM).
Cellular enzymatic changes of these arsenic-induced biochemical changes must be tested further by using in vivo and in vitro animal models.
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