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Subsequently to induce GFPmut2, 1 or 10 mM arabinose (HiMedia, India) was added to the wells that were to be tested for induction.
Also, kanamycin was added to the wells that were to be tested for induction in the presence of the antibiotic, to a final concentration of 10 μg/ml.
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As the transfected vector encodes a flag-tagged NBPF1 fusion protein, single colonies were tested for induction of NBPF1 expression by immunofluorescence staining with the anti-flag antibody (Sigma).
These fibrils were tested for induction of intracellular amyloid inclusion formation as previously described [ 38, 39].
However, no studies have been reported in which low levels of d-glucose were tested for induction of the amylolytic system in Aspergillus.
Further, candidates were tested for induction during ripening in 'Elyana' (only ripe fruits have detectable γ-D), and two environments with low or high γ-D production.
It is abundantly and specifically expressed in midgut during multiple developmental stages [ 60], and is weakly induced in larvae by bacterial challenge, though it has not been tested for induction in Plasmodium-infected mosquitoes.
In a later study, eight citrus limonoids were tested for induction of GST activity in mice and the structural features of different citrus limonoids and their effect on GST activity was reported [ 21].
To further determine the functional role of AP-1 transcription factor in BK-induced MMP-9 promoter induction, wild-type (WT) and point-mutated (mt -AP1 mt -AP1romoter constructs (as illustrated in Figure 7C, upper part) were tested for induction by BK.
After thawing, the blasts were suspended in Stem Span medium (Stem Cell Technologies, Vancouver, BC, Canada) before incubation with agents to be tested for death induction.
After confirming expression of mCherry and GFP in each clone they must then be tested for the ability to quantify flux induction.
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